Phospholipase A2 activity during the replication cycle of the flavivirus West Nile virus

S Liebscher; RL Ambrose; TE Aktepe; A Mikulasova; JE Prier; LK Gillespie; AJ Lopez-Denman; TWT Rupasinghe; D Tull; MJ McConville; JM Mackenzie

Journal article

Phospholipase A2 activity during the replication cycle of the flavivirus West Nile virus

S Liebscher, RL Ambrose, TE Aktepe, A Mikulasova, JE Prier, LK Gillespie, AJ Lopez-Denman, TWT Rupasinghe, D Tull, MJ McConville, JM Mackenzie

Plos Pathogens | PUBLIC LIBRARY SCIENCE | Published : 2018

DOI: 10.1371/JOURNAL.PPAT.1007029

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Abstract

Positive-sense RNA virus intracellular replication is intimately associated with membrane platforms that are derived from host organelles and comprised of distinct lipid composition. For flaviviruses, such as West Nile virus strain Kunjin virus (WNVKUN) we have observed that these membrane platforms are derived from the endoplasmic reticulum and are rich in (at least) cholesterol. To extend these studies and identify the cellular lipids critical for WNVKUN replication we utilized a whole cell lipidomics approach and revealed an elevation in phospholipase A2 (PLA2) activity to produce lyso-phosphatidylcholine (lyso-PChol). We observed that the PLA2 enzyme family is activated in WNVKUN-infecte..

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University of Melbourne Researchers

Turgut Aktepe Author

Malcolm McConville Author

Jason Mackenzie Author

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Grants

Awarded by National Health and Medical Research Council

Funding Acknowledgements

This research was supported by Project Grants (No. 1004619 and 1081786) to JMM from the National Health and Medical Research Council of Australia (https://www.nhmrc.gov.au). MJM is a NHMRC Principal Research Fellow. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.